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orf expression clone for mrgprx2  (Genecopoeia)


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    Genecopoeia orf expression clone for mrgprx2
    Orf Expression Clone For Mrgprx2, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1645 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/orf+expression/ORF+expression+clone+for+MRGPRX2/custom%40ex-t3417-m35%4010%2E31083%2Ffbl51779
    Average 94 stars, based on 1645 article reviews
    orf expression clone for mrgprx2 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: Sodium Proton Exchanger NHE9 pHine-Tunes Exosome Production by Impairing Rab7 Activity.
    Article Snippet: .. The ORF expression clone for SLC9A9 (NM_173653.3) was obtained from GeneCopoeia. ..

    Article Title: NHE9 regulates exosomal stress response to hypoxia in cardiomyocytes.
    Article Snippet: Hypoxia, a major stressor in conditions like ischemia, significantly impacts the function and communication of cardiomyocytes.. Cells adapt to hypoxia through various mechanisms, including the release of exosomes.. Exosomes are nanoscale vesicles that facilitate intercellular signaling by transferring specific biomolecular cargos.

    Article Title: An mTORC1-mediated negative feedback loop constrains amino acid-induced FLCN-Rag activation in renal cells with TSC2 loss
    Article Snippet: For experiments involving amino acid starvation, cells were rinsed in PBS and incubated in amino acid-free DMEM (#MBS6120661, MyBioSource) supplemented with 10% dialyzed serum (#26400044, Gibco) for 60-90 min. For amino acid addback, cells were stimulated with a 1X mix of essential (#11130036, Thermo Fisher Scientific) and non-essential amino acids (#11140035, Thermo Fisher Scientific) and 200 mM L-Glutamine (#25030081, Thermo Fisher Scientific), added directly to starved cells for 30 min. Plasmids and Lentiviral transfections: The following plasmids were used: p-EGFP-N1TFEB (38119, Addgene), pEGFP-N1-delta30-TFEB (44445, Addgene), pEGFP-N1TFEB-S3A,R4A (44446, Addgene), p-EGFP-N1-TFE3 (38120, Addgene), pRK5-HA GST RagB wt (19301, Addgene), pRK5-HA GST RagB 54L (19302, Addgene), pRK5-HA GST RagB 99L (19303, Addgene), pRK5-HA GST RagC wt (19304, Addgene), pRK5-HA GST RagC 75L (19305, Addgene), pRK5-HA GST RagC 120L (19306, Addgene), pRK5-HA GST RagD 77L (19308, Addgene), [pSK729] pRK5 HA-FLCN (R164A) (136147, Addgene), N1-p18-EGFP (42334, Addgene), FLCN-eGFP (72289, Addgene), pRK5-HA-FNIP1 (72293, Addgene), pRK5-HA-FNIP2 (72295, Addgene), pcDNA3-HA14-3-3 gamma (13274, Addgene), 4XCLEAR-luciferase reporter (66800, Addgene), pLKO.1 scramble shRNA (1864, Addgene), pLKO.1 FLCN lentiviral shRNA (TRCN0000237886, RNAi Consortium, Sigma), pLKO.1 FNIP2 lentiviral shRNA (TRCN0000256305; TRCN0000256306, RNAi Consortium, Sigma) and pLKO.1 RRAGD lentiviral shRNA (30321, Addgene). .. An ORF expression clone for human FNIP2 (NM_020840.3) in a lentiviral vector with N-eGFP and puromycin resistance was custom produced by GeneCopoeia. .. Primary antibodies: TSC1 (6935, Cell Signaling), 1:2000; TSC2 (4308, Cell Signaling), 1:2000; Raptor (2280, Cell Signaling), 1:1000; Phospho-p70 S6 Kinase (Thr389) (9205, Cell Signaling), 1:1000; p70 S6 Kinase (9202, Cell Signaling), 1:1000; Phospho-S6 Ribosomal Protein (Ser235/236) (4858, Cell Signaling), 1:2000; S6 Ribosomal Protein (2317, Cell Signaling), 1:2000; Phospho-4E BP1 (Ser65) (9451, Cell Signaling), 1:2000; Phospho-4E BP1 (Thr37/46) (2855, Cell Signaling), 1:1000; 4E-BP1 (9644, Cell Signaling), 1:2000; β-Actin (3700, Cell Signaling), 1:4000; Gapdh (2118, Cell Signaling), 1:4000; Phospho-Akt (S473) (4060, Cell Signaling), 1:1000; Akt (pan) (4691, Cell Signaling), 1:1000; LAMP-1 (9091, Cell Signaling), 1:2000; LAMP-2 (ABL93, DSHB at the University of Iowa), 1:50; CTSB (31718, Cell Signaling), 1:1000; CTSD (sc-6486, Santa Cruz), 1:500; CTSK (ab19027, Abcam), 1:1000; LC3A/B (12741, Cell Signaling), 1:1000; GPNMB (38313, Cell Signaling), 1:1000; p62/SQSTM1 (88588, Cell Signaling), 1:1000; Rab7 (9367, Cell Signaling), 1:1000; LAMTOR1 (8975, Cell Signaling), 1:1000; LAMTOR2 (8145, Cell Signaling), 1:1000; LAMTOR3 (8168, Cell Signaling), 1:1000; FLCN (3697, Cell Signaling), 1:1000; ; FNIP2 (57612, Cell Signaling), 1:1000; FNIP2 (HPA042779, Sigma), 1:1000; RagA (4357, Cell Signaling), 1:1000; RagB (8150, Cell Signaling), 1:1000; RagC (5466, Cell Signaling), 1:1000; RagD (4470, Cell Signaling), 1:1000; GABARAP (13733, Cell Signaling), 1:1000; TFEB (A303-673A, Bethyl), 1:500; TFE3 (PA5-54909, Thermo Fisher Scientific), 1:500; TFEB (4240, Cell Signaling), 1:1000; TFE3 (14779, Cell Signaling), 1:1000; Phospho-TFEB (S211) (37681, Cell Signaling), 1:500; Phospho-TFEB (S122) (86843, Cell Signaling), 1:500; Histone H3 (4499, Cell Signaling), 1:1000; Fibrillarin (2639, Cell Signaling), 1:1000; Lamin A/C (4777, Cell Signaling), 1:1000; Ki-67 (12202, Cell Signaling), 1:1000; GFP (55494, Cell Signaling), 1:1000; GFP (2956, Cell Signaling), 1:1000; HA (3724, Cell Signaling), 1:1000; FLAG (14793, Cell Signaling), 1:1000; 14-3-3 (pan) (8312, Cell Signaling), 1:1000.

    Article Title: Galectin-1 activates carbonic anhydrase IX and modulates glioma metabolism
    Article Snippet: At 450 nm, BrdU incorporation was determined using an xMark Bio-Rad microplate spectrophotometer. .. GST-tagged ORF expression clone for LGALS1 in bacteria was purchased from Genecopoeia (EX-I0309-B04). ..

    Article Title: Overexpression of GREM1 Improves the Survival Capacity of Aged Cardiac Mesenchymal Progenitor Cells via Upregulation of the ERK/NRF2-Associated Antioxidant Signal Pathway.
    Article Snippet: .. The bacterial stock of ORF expression clone (Catalog# EX-W0207-Lv105) for GREM1 (GeneCopoeia, Rockville, MD, USA) was used to grow bacterial colonies. .. After 12–16 h of growth, a single bacterial colony was picked from the agar dish and grown in liquid Luria broth (LB) culture, with vigorous shaking for 12–16 h. Maxiprep was performed using Qiagen kit to obtain plasmid DNA.

    Article Title: The relevance of EGFR, ErbB receptors and neuregulins in human adipocytes and adipose tissue in obesity.
    Article Snippet: The MISSION® siRNA Universal Negative Control #1 (Sigma-Aldrich, SIC001) was used as control in all experiments. .. ORF expression clone for NRG2 (217EX-W0006-M90-GS) and negative empty control vector for pReceiver-M90 (217EX-NEG-M90) were obtained from Genecopoeia ((Tebu-bio, Spain). .. Human subcutaneous preadipocytes were transfected with either NRG2 overexpression plasmid or control plasmid, using Fugene® Transfection Reagent (Promega, WI, USA) at ratio 1:3 following manufacturer’s instructions.

    Article Title: Sodium proton exchanger NHE9 pHine-tunes exosome production by impairing Rab7 activity
    Article Snippet: .. The ORF expression clone for SLC9A9 (NM_173653.3) was obtained from GeneCopoeia. .. Cells were cultured until approximately 70% confluency, then washed with PBS, and incubated in media containing exosome-depleted fetal bovine serum (A2720803, Thermo Fisher Scientific) for 48 h. Postincubation, the media was collected, and cell counts were recorded for normalization purposes.

    Over Expression:

    Article Title: NHE9 regulates exosomal stress response to hypoxia in cardiomyocytes.
    Article Snippet: Hypoxia, a major stressor in conditions like ischemia, significantly impacts the function and communication of cardiomyocytes.. Cells adapt to hypoxia through various mechanisms, including the release of exosomes.. Exosomes are nanoscale vesicles that facilitate intercellular signaling by transferring specific biomolecular cargos.

    Transfection:

    Article Title: NHE9 regulates exosomal stress response to hypoxia in cardiomyocytes.
    Article Snippet: Hypoxia, a major stressor in conditions like ischemia, significantly impacts the function and communication of cardiomyocytes.. Cells adapt to hypoxia through various mechanisms, including the release of exosomes.. Exosomes are nanoscale vesicles that facilitate intercellular signaling by transferring specific biomolecular cargos.

    Plasmid Preparation:

    Article Title: An mTORC1-mediated negative feedback loop constrains amino acid-induced FLCN-Rag activation in renal cells with TSC2 loss
    Article Snippet: For experiments involving amino acid starvation, cells were rinsed in PBS and incubated in amino acid-free DMEM (#MBS6120661, MyBioSource) supplemented with 10% dialyzed serum (#26400044, Gibco) for 60-90 min. For amino acid addback, cells were stimulated with a 1X mix of essential (#11130036, Thermo Fisher Scientific) and non-essential amino acids (#11140035, Thermo Fisher Scientific) and 200 mM L-Glutamine (#25030081, Thermo Fisher Scientific), added directly to starved cells for 30 min. Plasmids and Lentiviral transfections: The following plasmids were used: p-EGFP-N1TFEB (38119, Addgene), pEGFP-N1-delta30-TFEB (44445, Addgene), pEGFP-N1TFEB-S3A,R4A (44446, Addgene), p-EGFP-N1-TFE3 (38120, Addgene), pRK5-HA GST RagB wt (19301, Addgene), pRK5-HA GST RagB 54L (19302, Addgene), pRK5-HA GST RagB 99L (19303, Addgene), pRK5-HA GST RagC wt (19304, Addgene), pRK5-HA GST RagC 75L (19305, Addgene), pRK5-HA GST RagC 120L (19306, Addgene), pRK5-HA GST RagD 77L (19308, Addgene), [pSK729] pRK5 HA-FLCN (R164A) (136147, Addgene), N1-p18-EGFP (42334, Addgene), FLCN-eGFP (72289, Addgene), pRK5-HA-FNIP1 (72293, Addgene), pRK5-HA-FNIP2 (72295, Addgene), pcDNA3-HA14-3-3 gamma (13274, Addgene), 4XCLEAR-luciferase reporter (66800, Addgene), pLKO.1 scramble shRNA (1864, Addgene), pLKO.1 FLCN lentiviral shRNA (TRCN0000237886, RNAi Consortium, Sigma), pLKO.1 FNIP2 lentiviral shRNA (TRCN0000256305; TRCN0000256306, RNAi Consortium, Sigma) and pLKO.1 RRAGD lentiviral shRNA (30321, Addgene). .. An ORF expression clone for human FNIP2 (NM_020840.3) in a lentiviral vector with N-eGFP and puromycin resistance was custom produced by GeneCopoeia. .. Primary antibodies: TSC1 (6935, Cell Signaling), 1:2000; TSC2 (4308, Cell Signaling), 1:2000; Raptor (2280, Cell Signaling), 1:1000; Phospho-p70 S6 Kinase (Thr389) (9205, Cell Signaling), 1:1000; p70 S6 Kinase (9202, Cell Signaling), 1:1000; Phospho-S6 Ribosomal Protein (Ser235/236) (4858, Cell Signaling), 1:2000; S6 Ribosomal Protein (2317, Cell Signaling), 1:2000; Phospho-4E BP1 (Ser65) (9451, Cell Signaling), 1:2000; Phospho-4E BP1 (Thr37/46) (2855, Cell Signaling), 1:1000; 4E-BP1 (9644, Cell Signaling), 1:2000; β-Actin (3700, Cell Signaling), 1:4000; Gapdh (2118, Cell Signaling), 1:4000; Phospho-Akt (S473) (4060, Cell Signaling), 1:1000; Akt (pan) (4691, Cell Signaling), 1:1000; LAMP-1 (9091, Cell Signaling), 1:2000; LAMP-2 (ABL93, DSHB at the University of Iowa), 1:50; CTSB (31718, Cell Signaling), 1:1000; CTSD (sc-6486, Santa Cruz), 1:500; CTSK (ab19027, Abcam), 1:1000; LC3A/B (12741, Cell Signaling), 1:1000; GPNMB (38313, Cell Signaling), 1:1000; p62/SQSTM1 (88588, Cell Signaling), 1:1000; Rab7 (9367, Cell Signaling), 1:1000; LAMTOR1 (8975, Cell Signaling), 1:1000; LAMTOR2 (8145, Cell Signaling), 1:1000; LAMTOR3 (8168, Cell Signaling), 1:1000; FLCN (3697, Cell Signaling), 1:1000; ; FNIP2 (57612, Cell Signaling), 1:1000; FNIP2 (HPA042779, Sigma), 1:1000; RagA (4357, Cell Signaling), 1:1000; RagB (8150, Cell Signaling), 1:1000; RagC (5466, Cell Signaling), 1:1000; RagD (4470, Cell Signaling), 1:1000; GABARAP (13733, Cell Signaling), 1:1000; TFEB (A303-673A, Bethyl), 1:500; TFE3 (PA5-54909, Thermo Fisher Scientific), 1:500; TFEB (4240, Cell Signaling), 1:1000; TFE3 (14779, Cell Signaling), 1:1000; Phospho-TFEB (S211) (37681, Cell Signaling), 1:500; Phospho-TFEB (S122) (86843, Cell Signaling), 1:500; Histone H3 (4499, Cell Signaling), 1:1000; Fibrillarin (2639, Cell Signaling), 1:1000; Lamin A/C (4777, Cell Signaling), 1:1000; Ki-67 (12202, Cell Signaling), 1:1000; GFP (55494, Cell Signaling), 1:1000; GFP (2956, Cell Signaling), 1:1000; HA (3724, Cell Signaling), 1:1000; FLAG (14793, Cell Signaling), 1:1000; 14-3-3 (pan) (8312, Cell Signaling), 1:1000.

    Produced:

    Article Title: An mTORC1-mediated negative feedback loop constrains amino acid-induced FLCN-Rag activation in renal cells with TSC2 loss
    Article Snippet: For experiments involving amino acid starvation, cells were rinsed in PBS and incubated in amino acid-free DMEM (#MBS6120661, MyBioSource) supplemented with 10% dialyzed serum (#26400044, Gibco) for 60-90 min. For amino acid addback, cells were stimulated with a 1X mix of essential (#11130036, Thermo Fisher Scientific) and non-essential amino acids (#11140035, Thermo Fisher Scientific) and 200 mM L-Glutamine (#25030081, Thermo Fisher Scientific), added directly to starved cells for 30 min. Plasmids and Lentiviral transfections: The following plasmids were used: p-EGFP-N1TFEB (38119, Addgene), pEGFP-N1-delta30-TFEB (44445, Addgene), pEGFP-N1TFEB-S3A,R4A (44446, Addgene), p-EGFP-N1-TFE3 (38120, Addgene), pRK5-HA GST RagB wt (19301, Addgene), pRK5-HA GST RagB 54L (19302, Addgene), pRK5-HA GST RagB 99L (19303, Addgene), pRK5-HA GST RagC wt (19304, Addgene), pRK5-HA GST RagC 75L (19305, Addgene), pRK5-HA GST RagC 120L (19306, Addgene), pRK5-HA GST RagD 77L (19308, Addgene), [pSK729] pRK5 HA-FLCN (R164A) (136147, Addgene), N1-p18-EGFP (42334, Addgene), FLCN-eGFP (72289, Addgene), pRK5-HA-FNIP1 (72293, Addgene), pRK5-HA-FNIP2 (72295, Addgene), pcDNA3-HA14-3-3 gamma (13274, Addgene), 4XCLEAR-luciferase reporter (66800, Addgene), pLKO.1 scramble shRNA (1864, Addgene), pLKO.1 FLCN lentiviral shRNA (TRCN0000237886, RNAi Consortium, Sigma), pLKO.1 FNIP2 lentiviral shRNA (TRCN0000256305; TRCN0000256306, RNAi Consortium, Sigma) and pLKO.1 RRAGD lentiviral shRNA (30321, Addgene). .. An ORF expression clone for human FNIP2 (NM_020840.3) in a lentiviral vector with N-eGFP and puromycin resistance was custom produced by GeneCopoeia. .. Primary antibodies: TSC1 (6935, Cell Signaling), 1:2000; TSC2 (4308, Cell Signaling), 1:2000; Raptor (2280, Cell Signaling), 1:1000; Phospho-p70 S6 Kinase (Thr389) (9205, Cell Signaling), 1:1000; p70 S6 Kinase (9202, Cell Signaling), 1:1000; Phospho-S6 Ribosomal Protein (Ser235/236) (4858, Cell Signaling), 1:2000; S6 Ribosomal Protein (2317, Cell Signaling), 1:2000; Phospho-4E BP1 (Ser65) (9451, Cell Signaling), 1:2000; Phospho-4E BP1 (Thr37/46) (2855, Cell Signaling), 1:1000; 4E-BP1 (9644, Cell Signaling), 1:2000; β-Actin (3700, Cell Signaling), 1:4000; Gapdh (2118, Cell Signaling), 1:4000; Phospho-Akt (S473) (4060, Cell Signaling), 1:1000; Akt (pan) (4691, Cell Signaling), 1:1000; LAMP-1 (9091, Cell Signaling), 1:2000; LAMP-2 (ABL93, DSHB at the University of Iowa), 1:50; CTSB (31718, Cell Signaling), 1:1000; CTSD (sc-6486, Santa Cruz), 1:500; CTSK (ab19027, Abcam), 1:1000; LC3A/B (12741, Cell Signaling), 1:1000; GPNMB (38313, Cell Signaling), 1:1000; p62/SQSTM1 (88588, Cell Signaling), 1:1000; Rab7 (9367, Cell Signaling), 1:1000; LAMTOR1 (8975, Cell Signaling), 1:1000; LAMTOR2 (8145, Cell Signaling), 1:1000; LAMTOR3 (8168, Cell Signaling), 1:1000; FLCN (3697, Cell Signaling), 1:1000; ; FNIP2 (57612, Cell Signaling), 1:1000; FNIP2 (HPA042779, Sigma), 1:1000; RagA (4357, Cell Signaling), 1:1000; RagB (8150, Cell Signaling), 1:1000; RagC (5466, Cell Signaling), 1:1000; RagD (4470, Cell Signaling), 1:1000; GABARAP (13733, Cell Signaling), 1:1000; TFEB (A303-673A, Bethyl), 1:500; TFE3 (PA5-54909, Thermo Fisher Scientific), 1:500; TFEB (4240, Cell Signaling), 1:1000; TFE3 (14779, Cell Signaling), 1:1000; Phospho-TFEB (S211) (37681, Cell Signaling), 1:500; Phospho-TFEB (S122) (86843, Cell Signaling), 1:500; Histone H3 (4499, Cell Signaling), 1:1000; Fibrillarin (2639, Cell Signaling), 1:1000; Lamin A/C (4777, Cell Signaling), 1:1000; Ki-67 (12202, Cell Signaling), 1:1000; GFP (55494, Cell Signaling), 1:1000; GFP (2956, Cell Signaling), 1:1000; HA (3724, Cell Signaling), 1:1000; FLAG (14793, Cell Signaling), 1:1000; 14-3-3 (pan) (8312, Cell Signaling), 1:1000.

    Bacteria:

    Article Title: Galectin-1 activates carbonic anhydrase IX and modulates glioma metabolism
    Article Snippet: At 450 nm, BrdU incorporation was determined using an xMark Bio-Rad microplate spectrophotometer. .. GST-tagged ORF expression clone for LGALS1 in bacteria was purchased from Genecopoeia (EX-I0309-B04). ..

    Control:

    Article Title: The relevance of EGFR, ErbB receptors and neuregulins in human adipocytes and adipose tissue in obesity.
    Article Snippet: The MISSION® siRNA Universal Negative Control #1 (Sigma-Aldrich, SIC001) was used as control in all experiments. .. ORF expression clone for NRG2 (217EX-W0006-M90-GS) and negative empty control vector for pReceiver-M90 (217EX-NEG-M90) were obtained from Genecopoeia ((Tebu-bio, Spain). .. Human subcutaneous preadipocytes were transfected with either NRG2 overexpression plasmid or control plasmid, using Fugene® Transfection Reagent (Promega, WI, USA) at ratio 1:3 following manufacturer’s instructions.



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